Improvement on the competitive binding assay for the measurement of cyclic AMP by using ammonium sulphate precipitation.
نویسندگان
چکیده
The protein-binding assay developed by Brown, Albano, Ekins, Sgherzi & Tampion [(1971) Biochem. J. 121, 561-562] and Brown, Ekins & Albano [(1972) Adv. Cyclic Nucleotide Res. 2, 25-40] was modified by using precipitation with (NH4)2SO4 of the protein-cyclic AMP complex instead of adsorption of the free nucleotide on charcoal. The half-life of the protein-cyclic AMP complex obtained in the presence of charcoal was lower than that of the (NH4)2SO4-precipitated complex. In consequence, owing to the great stability of the precipitated protein-cyclic AMP complex, this method allows more accurate and reproducible determinations.
منابع مشابه
Method for determination of cyclic AMP in plasma.
A simple procedure is described for measuring cyclic AMP (cAMP) in plasma, body fluids, and tissue extracts. A modification of the method of Brown et al. [Biochem. J. 121, 561 (1971)], it is based on competitive binding of CAMP by a protein in crude adrenal extracts, and precipitation of the cAMP-protein complex by ammonium sulfate. cAMP concentrations in normal human plasma ranged from 8 to 16...
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عنوان ژورنال:
- The Biochemical journal
دوره 245 3 شماره
صفحات -
تاریخ انتشار 1987